Author: 1
Company: Microsoft

Specialization: 510406 Biophysics.
Curriculum: 18 Molecular Biophysics.
Supervisor of the curriculum: Prof. Trusov A. A.
Department of molecular biophysics.
Scientific adviser: Candidate of science Moroshkina E. B.
Reviewer: Candidate of science Polyanichko A. M.

The influence of the heterocyclic system size on the interaction of isoquinoline derivatives with DNA

Leontyeva Tatiana Vasilievna.

Several ligands belongs to alkaloid’s class with chromophores consist of fused ring system of 2-4 aromatic rings were investigated in this paper. The aim of paper was a possibility and characteristics of intercalation compounds to duplex of DNA. Investigations realized by using indirect methods: absorption spectroscopy, circular dichroism and viscosimetry. During this work were revealed number of binding way, thermodynamic parameters of binding (binding constant k and number of binding sites n), type of binding, where it was possible, and change in thermodynamic rigidity for DNA after complexation with ligand (for intercalating agents). It was investigated that (1) ligans with plat heterocycliс chromophore consist of 2-4 rings can intercalate into a duplex independently of its size (2) possibility of intercalation strongly depends on nature and position of substitutes (3) binding constant of intercalation and thermodynamic rigidity rise together with the number of fused aromatiс rings.

Publication’s list:

1. Interaction of the molecule DNA with coralyne and papaverine derivatives. Leontyeva T. V., Moroshkina E. B., Krivorotov D. V. – Collected thesis of III International conference “Modern problems of molecular biophysics”, Saint-Petersburg, 2011.
2. Papaverine in the synthesis of new DNA ligands. Krivorotov D. V., Moroshkina E. B., Leontyeva T. V. – Proceedings of the VI Moscow international congress “Biotechnology: State of the art and prospect of development”, Moscow, 2011.