Main field of study: 011200 Physics
Area of specialization: 09 Molecular Biophysics
Department of Molecular Biophysics and Polymer Physics
Scientific supervisor: PhD, Associate Professor A.M. Polyanichko
Reviewer: PhD А.S. Tsimokha

Interaction of diamminedichloroplatinum(II) with DNA and proteins
I.A. Belaya

Cis-diamminedichloroplatinum (cis-DDP) is one of the most successful anti-tumor drugs. The main target of cis-DDP is DNA. The biological activity of cis-DDP involves forming intrastrand adduct by binding to N7 atoms of adjacent purine DNA bases, which leads to cellular apoptosis. Trans-DDP, which is clinically ineffective, doesn’t form such type of intrastrand adduct. Although DNA is believed to be the major target of cis-DDP, the biological activity of the drug may be associated with nuclear proteins, such as histones, which are responsible for the first level of packing DNA.
The work is devoted to the investigation of the mechanisms of action cis-DDP and trans-DDP with DNA and proteins. The interaction of histones with DDP was investigated by the methods of Gel Electrophoresis and Analytical Ultracentrifugation. The ways of the binding DDP with DNA and proteins were investigated by IR spectroscopy. As a model protein for IR spectroscopy was chosen BSA.

The list of the publications
1. E.V. Chikhirzhina, M.S. Feofilova, I.A. Belaya, M.A. Ogarkov, E.I. Kostyleva, G.I. Chikhirzhina, A.M. Polyanichko. The structure of complexes of nuclear proteins HMGB1 and H1 with DNA modified by cisplatin// Book of abstract, 9th European Biophysics Congress. Portugal, 2013. P. 135. 
2. A.M. Polyanichko, I.A. Belaya, N.M. Romanov, E.I. Kostyleva, E.V. Chikhirzhina. Interaction of DNA with Nuclear Proteins and Diamminedichloroplatinum(II) Studied by Infrared Spectroscopy// Book of abstract , European Conference on the Spectroscopy of Biological Molecules. UK, 2013. P. 114.